For the quantitative determination of alkaline phosphatase in human serum using the Tokyo Boeki Medisys Inc. Biolis 24i analyzer. For in vitro diagnostic use only.
Principle
ì?-Nitrophenyl phosphate is hydrolyzed to ì?-nitrophenol and inorganic phosphate. The rate at which the ì?-NPP is hydrolyzed, measured at 405nm, is directly proportional to the alkaline phosphatase activity.
