Principle
ë±-Amylase hydrolyzes the 2-chloro-p-nitrophenyl-ë±-D-maltotrioside (CNPG3) to release 2-chloro-nitrophenol and form 2-chloro-p-nitrophenyl-ë±-D-maltoside (CNPG2), maltotriose (G3) and glucose (G). The rate of increase in absorbance is measured at 405 nm and is proportional to the ë±-amylase activity in the sample.