For the quantitative determination of Glucose in serum on the Tokyo Boeki Medisys Inc. Biolis 24i analyzer. For in vitro diagnostic use only.
Glucose is oxidized by glucose oxidase to gluconate and hydrogen peroxide. Phenol + 4-AAP + hydrogen peroxide, in the presence of peroxidase, produces a quinoneimine dye that is measured at 500nm. The absorbance at 500nm is proportional to the concentration of glucose in the sample.