Ammonia (A7553-85)

Ammonia (A7553-85)

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Intended Use
This reagent is intended for the enzymatic measurement of ammonia in plasma. For in vitro diagnostic use only.
Principle
The enzymatic determination of ammonia allows a direct measurement of the compound in the plasma which avoids the long and laborious methods of separation employed in older methodologies. The enzymatic assay gives a highly sensitive and specific method. Ammonia reacts with _-Ketoglutarate (_-KG) and reduced nicotinamide adenine dinucleotide phosphate (NADPH) to form L-glutamate and NADP in a reaction catalyzed by glutamate dehydrogenase (GLDH){L-glutamate: NAD(P) + oxidoreductase (deaminating), EC 1.4.1.3}. The amount of NADPH oxidized is, on a molar basis, equal to the content of ammonia in the sample. The reaction can be followed by the decrease in absorbance at 340nm. The reagent is provided in two separate vials. Keeping the components of the reagent separated until time of assay increases their stability after reconstitution. The use of NADPH in place of NADH minimizes interference by such components of plasma as pyruvate and lactate dehydrogenase.

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