Amylase (A7564-60)

Amylase (A7564-60)

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Intended Use
For the quantitative kinetic determination of _-amylase activity in human serum using a manual or automated procedure.
Principle
_-Amylase hydrolyzes the 2-chloro-p-nitrophenyl-_-D-maltotrioside (CNPG3) to release 2-chloro-nitrophenol and form 2-chloro-p-nitrophenyl-_-D-maltoside (CNPG2), maltotriose (G3) and glucose (G). The rate of increase in absorbance is measured at 405 nm and is proportional to the _-amylase activity in the sample.

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