Bilirubin, Total (B7576-1L)

Bilirubin, Total (B7576-1L)

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Intended Use
For the quantitative determination of total bilirubin in serum.
Principle
Sulfanilic acid reacts with sodium nitrite to produce diazotized sulfanilic acid (diazo). Direct and indirect bilirubin couple with diazo to produce azobilirubin in the presence of dimethyl sulfoxide (DMSO). The intensity of the color produced is directly proportional to the amount of total bilirubin concentration present in the sample.

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