For the quantitative determination of Glycohemoglobin (HbA1) in blood by cation exchange resin. The test is to be used to monitor long-term glucose control in Diabetes mellitus.
A hemolyzed preparation of the whole blood is mixed continuously for five minutes with a weak binding cation-exchange resin. During this time, HbA0 binds to the resin. After the mixing period, a filter is used to separate the supernatant containing the glycohemoglobin from the resin. (Note: This binding is temperature dependent. Therefore, a standard should be included in each run.) The percent glycohemoglobin is determined by measuring the absorbance at 415 nm (405-420 nm acceptable) of the glycohemoglobin fraction and the total hemoglobin fraction. The ratio of the two absorbances gives the percent glycohemoglobin.