For the quantitative, kinetic determination of glucose-6-phosphate dehydrogenase (G6PD) in blood at 340nm. For in vitro diagnostic use only.
Glucose-6-phosphate dehydrogenase (G6PD, D-glucose-6-phosphate: oxidoreductase, EC 188.8.131.52) catalyzes the first step in the pentose phosphate shunt, oxidizing glucose-6-phosphate (G-6-P) to 6-phosphogluconate (6-PG) and reducing NADP to NADPH. This procedure is a modification of the spectrophotometric methods of Kornberg and Horecker and of Lohr and Waller, involving the following reaction: Nictotinamide adenine dinucleotide phosphate (NADP) is reduced by G6PD in the presence of G-6-P. The rate of formation of NADPH is proportional to the G6PD activity and is measured spectrophotometrically as in increase in absorbance at 340nm. Production of a second molar equivalent of NADPH by erythrocyte 6-phosphogluconate dehydrogenase (6-PGDH) is prevented by use of maleimide, an inhibitor of 6-PGDH.