For the quantitative determination of High Density Lipoprotein (HDL) in Cholesterol in serum.
When serum is combined with the polyethylene glycol reagent, all beta-lipoproteins (LDL and VLDL) are precipitated. The HDL fraction (alpha-fraction) remains in the supernatant. The supernatant is then treated as a sample and assayed for cholesterol by an enzymatic method. The value obtained is the HDL cholesterol value.