B-Hydroxybutyrate(H7587-58)

B-Hydroxybutyrate(H7587-58)

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Intended Use
For the quantitative determination of _-hydroxybutyrate in serum or plasma. For in vitro diagnostic use only.
Principle
Enzymatic quantitation of _-hydroxybutyrate by _-hydroxybutyrate dehydrogenase has been reported. In the Pointe Scientific method, _-hydroxybutyrate (D-3-hydroxybutyrate) in the presence of NAD gets converted to acetoacetate and NADH at a pH 8.5 by _-hydroxybutyrate dehydrogenase (D-3-hydroxybutyrate dehydrogenase). At this pH, the reaction is favored to the right. The NADH produced is converted to color using INT and diaphorase.

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