For the quantitative determination of _-hydroxybutyrate in serum or plasma. For in vitro diagnostic use only.
Enzymatic quantitation of _-hydroxybutyrate by _-hydroxybutyrate dehydrogenase has been reported. In the Pointe Scientific method, _-hydroxybutyrate (D-3-hydroxybutyrate) in the presence of NAD gets converted to acetoacetate and NADH at a pH 8.5 by _-hydroxybutyrate dehydrogenase (D-3-hydroxybutyrate dehydrogenase). At this pH, the reaction is favored to the right. The NADH produced is converted to color using INT and diaphorase.