For the quantitative determination of alanine aminotransferase in serum on Hitachi analyzers. For in vitro diagnostic use only.
ALT catalyzes the transfer of the amino group from L-alanine to _-ketoglutarate resulting in the formation of pyruvate and L-glutamate. Lactate dehydrogenase catalyzes the reduction of pyruvate and the simultaneous oxidation of NADH to NAD. The resulting rate of decrease in absorbance is directly proportional to ALT activity.