For the quantitative kinetic determination of _-amylase activity in human serum. For in vitro diagnostic use only.
_-Amylase hydrolyzes the substrate (EPS-G7) to produce oligosaccharides. The _-glucosidase hydrolizes the oligosaccharides liberating _-nitrophenol. The rate of increase in absorbance is measured at 415nm and is proportional to the _-amylase activity in the sample.