For the quantitative determination of Glucose in serum on Hitachi analyzers. For in vitro diagnostic use only.
Glucose is oxidized by glucose oxidase to gluconate and hydrogen peroxide. Phenol + 4-AAP + hydrogen peroxide, in the presence of peroxidase, produces a quinoneimine dye that is measured at 500nm. The absorbance at 500nm is proportional to the concentration of glucose in the sample.