For the quantitative determination of triglycerides in serum or plasma on Hitachi analyzers. For in vitro diagnostic use only.
Serum triglycerides are hydrolyzed to glycerol and free fatty acids by lipase. In the presence of ATP and glycerol kinase (GK), the glycerol is converted to glycerol-1-phosphate. The glycerol-1-phosphate is then oxidized by glycerol phosphate oxidase (GPO) to yield hydrogen peroxide. The condensation of hydrogen peroxide with 4-chlorophenol and 4-aminophenazone (4-AA) in the presence of peroxidase (POD) produces a red colored quinoneimine dye which absorbs at, or near 505nm. The intensity of the colored complex formed is directly proportional to the triglycerides concentration of the sample.