For the quantitative determination of uric acid in serum on Hitachi analyzers. For in vitro diagnostic use only.
Uric acid is oxidized by uricase to allantoin and hydrogen peroxide. HDCBS + 4-AAP + hydrogen peroxide, in the presence of peroxidase, produces a red chromogen that is measured at 520 nm. The absorbance is proportional to the concentration of uric acid in the sample.