For the quantitative determination of lactate in human plasma. For in vitro diagnostic use only.
Lactate oxidase catalyzes the oxidation of lactic acid to pyruvate and hydrogen peroxide. Peroxidase then catalyzes the reaction of hydrogen peroxide with a hydrogen donor, in the presence of 4-aminophenazone, to form a dye. Color intensity, measured at 550nm, is proportional to the lactate concentration in the sample.