Triglycerides (T7531-150)

Triglycerides (T7531-150)

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Intended Use
For the quantitative determination of triglycerides in serum or plasma.
Principle
Triglycerides in the sample are hydrolyzed by lipase to glycerol and fatty acids. The glycerol is then phosphorylated by adenosine-5-triphosphate (ATP) to glycerol-3-phosphate (G3P) and adenosine-5-diphosphate in a reaction catalyzed by glycerol kinase (GK). Glycerol-3-phosphate is then converted to dihydroxyacetone phosphate (DAP) and hydrogen peroxide by glycerophosphate oxidase (GPO). The hydrogen peroxide then reacts with 4-aminoantipyrine (4-AAP) and 3-hydroxy-2,4,6-tribomobenzoic acid (TBHB) in a reaction catalyzed by peroxidase to yield a red colored quinoneimine dye. The intensity of the color produced is directly proportional to the concentration of Triglycerides in the sample when measured at 540nm.

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