For the quantitative determination of Uric Acid in serum. For in vitro diagnostic use only.
Uric Acid is oxidized by Uricase to allantoin and hydrogen peroxide. HDCBS + 4-AAP + hydrogen peroxide, in the presence of peroxidase, produces a red chromagen that is measured at 520nm. The absorbance at 520nm is proportional to the concentration of Uric Acid in the sample.